여행과 삶

쾰른 크리스마스 마켓 구경하고 발렌시아로 떠나기

쾰른 크리스마스 마켓 유럽의 연말은 마치 한국의 설날과 비슷하다. 대부분의 사람들이 크리스마스에 맞춰서 자신의 부모님 집에 방문하는 것과 같이 가족과 함께하는 날이다. 따라서 크리스마스 이후로 약 7~10일정도 휴가를 갖는 것이 일반적이다. 독일에서는 크리스마스 당일인 25일 뿐만 아니라 26일도 휴일이다. 2022년의 크리스마스는 25일이 일요일이었는데, 독일 정부에서 23일인 금요일도 국가 공휴일로 지정해 주었다. 따라서 금, 토, 일, 월요일에 이르는 연휴를 선물 받았고, 나는 뒤에 연가를 붙여 금요일까지 휴가를 사용하였다. 이번 크리스마스에는 스페인 발렌시아에 방문하기로 하였다. 역시 크리스마스 연휴에는 비행기 표의 가격이 매우 비쌌다. 저가 항공인 라이언 에어 항공기를 이용하였고, 왕복 약 35..

2023. 10. 1. 17:43

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[생물공학실험] The basics of Animal cell culture and calculation doubling time

Animal cell culture Most biomedical drugs are antibodies or proteins from animal cells. If biomedical proteins are injected into the human body, they are easily broken down and removed by the human defense system. Therefore, not only the structure of the protein but also the glycosylation, which is made up of carbohydrates, is very important. To apply this glycosylation as close as possible to t..

2023. 10. 1. 06:30

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[생물공학실험] Enzyme kinetics for Michaelis-Menten equation and Lineweaver-Burk graph

Enzyme kinetics The study of enzyme kinetics is the ground of enzyme production for industry. Actually, a lot of Enzymes are applied and produced with mass production. Therefore, It is very important to understand enzyme reaction kinetics and the kinetics are based on the “Michaelis-Menten equation and Lineweaver-Burk graph“. Michaelis-Menten equation: V0 = Vmax * [s] / (km + [s]) # Lineweaver-B..

2023. 10. 1. 06:24

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[생물공학실험] Gram-Staining (Gram-negative and -positive) with using Chemicals

Gram Staining (Gram-negative and -positive) Gram-staining is useful to distinguish certain Microbes, which mostly consist of gram-positive and gram-negative. For the classification of microorganisms, gram-staining is used with each character, in which Gram-positive microbes have a big cell wall with peptidoglycan, and gram-negative have a thin cell wall with peptidoglycan. The Gram-negative/-pos..

2023. 10. 1. 06:18

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[생물공학실험]Inoculation and Culture for Microbes (Streaking and Spreading)

Inoculation and Culture for Microbes Inoculation and culture for Microbes for streaking and spreading is the basest technology when experimenting with microbes. Microorganisms always exist as different kinds of mixtures in nature. To pick out and study just one type of microorganism in microbiology, therefore, one type of microorganism has to be sorted by using a streaking method. The stacking m..

2023. 10. 1. 06:12

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[생물공학실험] E. Coli Culture, LB Media Preparation And Sterilization

E. Coli Culture, LB Media & Sterilization E. coli is most commonly used in biotechnology to produce proteins or biological materials. The most commonly used growth medium for E. coli is LB broth (Luria-Bertani broth). To successfully cultivate E. coli, this LB broth contains some growth vectors, vitamins, amino acids, and metabolic energy sources such as glucose. In the growth media, undesired m..

2023. 10. 1. 06:07